Table 1 The designed primer sequences used for PCR amplification and RT-PCR in tilapia species
Gene name | Forward sequence | Sequence and RT-PCR | NCBI reference sequence |
|---|---|---|---|
Hepcidin 1 | 5′-ACCAGGAGAAAGAGTCAAAC-3′ 5′-ACAGTATAATCTCACACATTGTC-3′ | Sequence | XM_003456839.1 |
Hepcidin 2 | 5′-TCTTCAAAGGGTACAACAAC-3′ 5′-AGAAAGAGTCAGACAATAAGC-3′ | Sequence | XM_003450530.4 |
Hepcidin 1 | 5′-CAG CAG CAC ATG AGG AGG CAT-3′ 5′-ACC TGC AGC AAA CTC CAC AGA-3′ | RT-PCR | MH651353.1 |
Hepcidin 2 | 5′-AGA GCA ATG ACA CTC CAG TTG-3′ 5′-AGA TGG CTC TGA CGC TTT TG-3′ | RT-PCR | MH651359.1 |
β-Actin | 5′-CGG AAT CCA CGA AAC CAC CTA-3′ 5′-TTG CTG ATC CAC ATC TGC TGG-3′ | RT-PCR | AB037865.1 |