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Table 1 The designed primer sequences used for PCR amplification and RT-PCR in tilapia species

From: Molecular characterization and expression analysis of hepc1 and hepc2 in three tilapia species collected from Lake Manzala

Gene nameForward sequenceSequence and RT-PCRNCBI reference sequence
Hepcidin 15′-ACCAGGAGAAAGAGTCAAAC-3′
5′-ACAGTATAATCTCACACATTGTC-3′
SequenceXM_003456839.1
Hepcidin 25′-TCTTCAAAGGGTACAACAAC-3′
5′-AGAAAGAGTCAGACAATAAGC-3′
SequenceXM_003450530.4
Hepcidin 15′-CAG CAG CAC ATG AGG AGG CAT-3′
5′-ACC TGC AGC AAA CTC CAC AGA-3′
RT-PCRMH651353.1
Hepcidin 25′-AGA GCA ATG ACA CTC CAG TTG-3′
5′-AGA TGG CTC TGA CGC TTT TG-3′
RT-PCRMH651359.1
β-Actin5′-CGG AAT CCA CGA AAC CAC CTA-3′
5′-TTG CTG ATC CAC ATC TGC TGG-3′
RT-PCRAB037865.1