Fig. 5

Influence of different pH values on bactericidal activity of sodium benzoate against E. coli O157:H7, S. enteritidis, and L. monocytogenes. Sodium benzoate concentrations (0, 5, 10, 15, 20, and 25) ppm were each added to 10-ml tryptone soya broth tubes and then inoculated (50 μl) of an overnight culture of bacterial suspension (O.D 600 = 1.818). Tubes were incubated at 37 °C for 24 h. Negative and positive controls were performed. The optical density of each tube was measured using a spectrophotometer. Data shown as a mean of two independent experiments with standard error (P < 0.05)