PCR round | Primer | Primer sequence | Time |
---|---|---|---|
1st round | Crtd-A (forward primer) | 5′-GGTGGAGGTTCTTGTCTTGGTA-3′ | 57.5 °C |
Crtd-B (reverse primer) | 5′-GACCTATGAAGGCCAAAATGACTG-3′ | 56.7 °C | |
2nd round | Crtd-C (forward primer) | 5′-TGTGCTCATGTGTTTAAACTT-3′ | 50.0 °C |
Crtd-D (reverse primer) | 5′-CAAAACTATAGTTACCAATTTT-3′ | 46.1 °C |