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Table 2 Effects of freezing protocols on frozen thawed rooster semen cryopreserved with coconut water extender containing different concentrations of glycerol and DMSO

From: Comparison of conventional and automated freezing methods on PB2 rooster semen cryopreserved with glycerol and dimethylsulfoxide tris coconut-water extender

   Freezing Protocols     
Cryoprotectants Concentrations Parameters FP1 FP2 FP3 FP4 SEM
Glycerol (8%) Motility (%) 73.33a 56.68b 50.00b 23.33c 5.57
Viability (%) 68.33ab 72.00a 67.67ab 56.33b 2.49
Host (%) 67.67 67.67 59.33 59.33 2.84
(10%) Motility (%) 43.33ab 48.33a 40.00b 36.67b 1.56
Viability (%) 68.33 71.67 63.67 74.38 1.79
Host (%) 56.67 64.00 58.00 62.67 2.25
(15%) Motility (%) 26.67c 38.33b 33.33bc 71.67a 5.31
Viability (%) 65.00ab 63.67ab 58.00b 76.33a 2.66
Host (%) 51.33ab 60.00ab 50.67b 71.67a 3.63
DMSO (8%) Motility (%) 26.67c 35.00bc 40.00ab 50.00a 2.98
Viability (%) 64.67b 65.67b 54.67b 82.00a 3.49
Host (%) 57.67bc 64.33ab 45.00c 76.33a 3.99
(10%) Motility (%) 71.67b 75.00ab 76.67a 78.33a 0.97
Viability (%) 76.33b 82.00ab 80.67ab 83.67a 1.19
Host (%) 77.33 81.67 77.33 84.33 1.51
(15%) Motility (%) 70.00 70.00 71.33 71.67 0.65
Viability (%) 77.33b 84.33ab 88.00a 81.67ab 1.66
  Host (%) 75.00b 81.33ab 83.33a 79.33ab 1.37
  1. Superscripts indicate significant difference at 5% level within the columns (a–c)
  2. SEM standard error of mean for freezing protocols