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Table 2 Effects of freezing protocols on frozen thawed rooster semen cryopreserved with coconut water extender containing different concentrations of glycerol and DMSO

From: Comparison of conventional and automated freezing methods on PB2 rooster semen cryopreserved with glycerol and dimethylsulfoxide tris coconut-water extender

  

Freezing Protocols

    

Cryoprotectants

Concentrations

Parameters

FP1

FP2

FP3

FP4

SEM

Glycerol

(8%)

Motility (%)

73.33a

56.68b

50.00b

23.33c

5.57

Viability (%)

68.33ab

72.00a

67.67ab

56.33b

2.49

Host (%)

67.67

67.67

59.33

59.33

2.84

(10%)

Motility (%)

43.33ab

48.33a

40.00b

36.67b

1.56

Viability (%)

68.33

71.67

63.67

74.38

1.79

Host (%)

56.67

64.00

58.00

62.67

2.25

(15%)

Motility (%)

26.67c

38.33b

33.33bc

71.67a

5.31

Viability (%)

65.00ab

63.67ab

58.00b

76.33a

2.66

Host (%)

51.33ab

60.00ab

50.67b

71.67a

3.63

DMSO

(8%)

Motility (%)

26.67c

35.00bc

40.00ab

50.00a

2.98

Viability (%)

64.67b

65.67b

54.67b

82.00a

3.49

Host (%)

57.67bc

64.33ab

45.00c

76.33a

3.99

(10%)

Motility (%)

71.67b

75.00ab

76.67a

78.33a

0.97

Viability (%)

76.33b

82.00ab

80.67ab

83.67a

1.19

Host (%)

77.33

81.67

77.33

84.33

1.51

(15%)

Motility (%)

70.00

70.00

71.33

71.67

0.65

Viability (%)

77.33b

84.33ab

88.00a

81.67ab

1.66

 

Host (%)

75.00b

81.33ab

83.33a

79.33ab

1.37

  1. Superscripts indicate significant difference at 5% level within the columns (a–c)
  2. SEM standard error of mean for freezing protocols