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Table 6 Band variation and polymorphism percentage in seven wheat lines using the six ISSR primers

From: Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation

Primers Total bands Molecular size (bp) Number of monomorphic Number of unique band (positive marker) Number of polymorphic Polymorphism % Sequence Annealing
17898-A 29 1525–214 0 9 20 100% 5′-(CA)6 AC-3′ 38 °C
17899-A 26 2540–176 2 4 20 92.308% 5′-(CA)6 AG-3′ 38 °C
17898-B 24 2173–148 2 7 15 91.667% 5′-(CA)6 GT-3′ 40 °C
17899-B 37 2381–161 5 10 22 86.486% 5′-(CA)6 GG-3′ 41 °C
844-B 37 1915–164 1 19 17 97.297% 5′-(CT)8 GC-3′ 45 °C
HB-14 20 1262–190 2 7 11 90% 5′-(CTC)3 GC-3′ 38 °C
Total 173 2540–148 12 56 105 93%