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Table 6 Band variation and polymorphism percentage in seven wheat lines using the six ISSR primers

From: Elicitation of salt stress-tolerant mutants in bread wheat (Triticum aestivum L.) by using gamma radiation

Primers

Total bands

Molecular size (bp)

Number of monomorphic

Number of unique band (positive marker)

Number of polymorphic

Polymorphism %

Sequence

Annealing

17898-A

29

1525–214

0

9

20

100%

5′-(CA)6 AC-3′

38 °C

17899-A

26

2540–176

2

4

20

92.308%

5′-(CA)6 AG-3′

38 °C

17898-B

24

2173–148

2

7

15

91.667%

5′-(CA)6 GT-3′

40 °C

17899-B

37

2381–161

5

10

22

86.486%

5′-(CA)6 GG-3′

41 °C

844-B

37

1915–164

1

19

17

97.297%

5′-(CT)8 GC-3′

45 °C

HB-14

20

1262–190

2

7

11

90%

5′-(CTC)3 GC-3′

38 °C

Total

173

2540–148

12

56

105

93%

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