Assessment of semen characteristics and in vivo conception rate of preserved buffalo bull semen extended in tris enhanced with Diospyros kaki

El-Sheshtawy, Reda Ibrahim; El-Nattat, Walid Said

doi:10.1186/s42269-020-00353-5

Bulletin of the National Research Centre

Table 1 Effect of Diospyros kaki-enhanced extender on buffalo bull sperm motility during chilling

From: Assessment of semen characteristics and in vivo conception rate of preserved buffalo bull semen extended in tris enhanced with Diospyros kaki

Treatment	Time (2 h)	Days							F value	P<
Treatment	Time (2 h)	1	2	3	4	5	6	7	F value	P<
Control	90.00^aA ± 0.00	80.00^bAB ± 0.00	73.33^cdBC ± 3.33	60.00^dC ± 5.77	45.00^cD ± 10.41	20.00^dE ± 2.89	1.67^cF ± 1.67	0.00^cF ± 0.00	61.32	0.0001
1%	90.00^aA ± 0.00	80.00^bB ± 0.00	70.00^dC ± 0.00	68.33^bcC ± 1.67	56.67^abcD ± 1.67	33.33^abE ± 1.67	8.33^bcF ± 1.67	0.00^cG ± 0.00	383.64	0.0001
2%	90.00^aA ± 0.00	85.00^aA ± 0.00	70.00^dB ± 0.00	66.67^cdB ± 3.33	55.00^abcC ± 2.89	30.00^bcD ± 2.89	8.33^bcE ± 1.67	0.00^cE ± 0.00	243.38	0.0001
3%	91.67^aA ± 1.67	85.00^aB ± 0.00	81.67^abBC ± 1.67	78.33^aC ± 1.67	66.67^aD ± 1.67	41.67^aE ± 1.67	23.33^aF ± 1.67	6.67^abG ± 3.33	492.19	0.0001
4%	88.33^aA ± 1.67	85.00^aAB ± 0.00	81.67^abB ± 1.67	76.67^abC ± 1.67	63.33^aD ± 1.67	41.67^aE ± 1.67	25.00^aF ± 2.89	10.00^aG ± 2.89	375.70	0.0001
5%	90.00^aA ± 2.89	85.00^aB ± 0.00	81.67^abB ± 1.67	76.67^abC ± 1.67	63.33^aD ± 1.67	41.67^aE ± 1.67	20.00^aF ± 0.00	3.33^bcG ± 1.67	456.14	0.0001
6%	88.33^aA ± 1.67	85.00^aA ± 0.00	81.67^abAB ± 1.67	76.67^abB ± 1.67	55.00^abcC ± 2.89	30.00^bcD ± 2.89	11.67^bE ± 3.33	0.00^cF ± 0.00	275.42	0.0001
7%	88.33^aA ± 1.67	85.00^aA ± 0.00	85.00^aA ± 0.00	81.67^aA ± 1.67	60.00^abB ± 2.89	33.33^abC ± 4.41	10.00^bD ± 2.89	0.00^cE ± 0.00	251.39	0.0001
8%	88.33^aA ± 1.67	85.00^aA ± 0.00	76.67^bcB ± 1.67	73.33^abcB ± 1.67	48.33^bcC ± 1.67	25.00^bcdD ± 2.89	10.00^bE ± 2.89	0.00^cF ± 0.00	356.80	0.0001
9%	88.33^aA ± 1.67	85.00^aAB ± 0.00	80.00^abB ± 0.00	73.33^abcC ± 1.67	48.33^bcD ± 1.67	23.33^cdE ± 1.67	6.67^bcF ± 1.67	0.00^cF ± 0.00	386.03	0.0001
10%	88.33^aA ± 1.67	85.00^aAB ± 0.00	80.00^abBC ± 0.00	73.33^abcC ± 1.67	55.00^abcD ± 5.00	25.00^bcdE ± 2.89	8.33^bcF ± 3.33	0.00^cG ± 0.00	205.64	0.0001
F value	0.52	Infty	11.49	5.94	3.01	8.87	9.81	5.90
P<	0.8578	0.0001	0.0001	0.0002	0.0149	0.0001	0.0001	0.0003

Mean ± S.E.
Different small letter superscripts indicate a significant difference between means within column (for treatment) using the multiple range Duncan’s test at P < 0.05
Different capital letter superscripts indicate a significant difference between means within row (for time) using the multiple range Duncan’s test at P < 0.05

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