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Fig. 5 | Bulletin of the National Research Centre

Fig. 5

From: Potent bioactive methanolic extract of wild orange (Citrus macroptera Mont.) shows antioxidative, anti-inflammatory, and antimicrobial properties in in vitro, in vivo, and in silico studies

Fig. 5

Effects of CML in depletion of intracellular ROS production generated by H2O2 in HepG2 cells. Production of ROS was measured by cleavage of acetate group of non-fluorescent H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) which convert into DCF(2′,7′-dichlorofluorescein) highly fluorescent. Cells were exposed to H2O2 before treatment with CML at 200 μg/ml for 24 h. The ROS production displays the intensity of fluorescence through the images of HepG2 cells treated with different concentrations of CML: a control, b H2O2, and c CML extract

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