Fig. 5From: Potent bioactive methanolic extract of wild orange (Citrus macroptera Mont.) shows antioxidative, anti-inflammatory, and antimicrobial properties in in vitro, in vivo, and in silico studiesEffects of CML in depletion of intracellular ROS production generated by H2O2 in HepG2 cells. Production of ROS was measured by cleavage of acetate group of non-fluorescent H2DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) which convert into DCF(2′,7′-dichlorofluorescein) highly fluorescent. Cells were exposed to H2O2 before treatment with CML at 200 μg/ml for 24 h. The ROS production displays the intensity of fluorescence through the images of HepG2 cells treated with different concentrations of CML: a control, b H2O2, and c CML extractBack to article page