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Table 2 Effect of different type of nutrient media on percentage of calli induction calli fresh, and dry weights (g/jar) induced from internode explants of Diospyros kaki L. cv. Costataa

From: Biotechnological and chemical analysis of Egyptian Diospyros kaki L. cv. Costata grown in Egypt

No.

Mediab

% of callus induction

Calli fresh weight (g/jar)

Calli dry weight (g/jar)

1

MS-free growth regulators

2

WPM + 2 mg/l Zt + 5 mg/l 2iP

30 ± 4.15

0.68 ± 0.32

0.11 ± 0.06

3

MS + 10 mg/l Zt + 10 mg/l IAA + 500 mg/l PVP + 0.1 mg/l thiamine HCL

60 ± 5.25

6.69 ± 0.79

0.38 ± 0.11

4

½ MS + 1 mg/l BAp + 0.1 mg/l NAA + 1 mg/l IAA + 0.1 mg/l thiamine HCL

10 ± 2.33

5

½ MS + 0.5 mg/l BAp + 5 mg/l 2,4-D + 1 mg/l IAA + 0.1 mg/l thiamine HCl

40 ± 4.25

3.40 ± 0.75

0.13 ± 0.05

  1. aCultures were incubated under dark condition for four weeks at 26 ± 1 °C
  2. bEach treatment was the mean of 10 replicates ± SE