Stage | Volume (ml) | Protein concentration (mg/ml) | Specific activity (U/mg) | Total activity (U) | Purification factor (fold) | Yield % |
---|---|---|---|---|---|---|
Culture supernant | 900 | 5.3 | 6.6 | 31,500 | 1 | 100 |
Ammonium sulfate precipitation | 125 | 3.9 | 7.6 | 3750 | 1.151 | 11.90 |
Sephadex LH-G20 | 8 | 0.02 | 1750 | 280 | 230 | 0.88 |