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Table 2 Summary of the purification procedure of killer toxin from Wickerhamomyces anomalus

From: Purification and characterization of two killer toxins originated from Torulaspora delbrueckii (Lindner) and Wickerhamomyces anomalus (E.C.Hansen) Kurtzman, Robnett, and Basehoar-Powers

Stage

Volume (ml)

Protein concentration (mg/ml)

Specific activity (U/mg)

Total activity (U)

Purification factor (fold)

Yield %

Culture supernant

900

5.3

6.6

31,500

1

100

Ammonium sulfate precipitation

125

3.9

7.6

3750

1.151

11.90

Sephadex LH-G20

8

0.02

1750

280

230

0.88