Table 1 Summary of the purification procedure of killer toxin from Torulaspora delbrueckii
Stage | Volume (ml) | Protein concentration (mg/ml) | Specific activity (U/mg) | Total activity (U) | Purification factor (fold) | Yield % |
|---|---|---|---|---|---|---|
Culture supernatant | 900 | 5 | 6 | 27,000 | 1 | 100 |
Ammonium sulfate precipitation | 125 | 4.1 | 6.8 | 3500 | 1.133 | 12.9 |
Dialysis | 134 | 0.04 | 750 | 4020 | 110 | 14.8 |