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Table 3 Statistical analysis of CCD showing coefficients, t- values, P-values and standard errors

From: Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application

Term Coefficient estimate Std Error t ratio P value Prob > F VIF
Intercept 57.3542 0.78473 73.09 < 0.0001* 1
W: PEI % 1.7389 0.42836 4.06 0.0023* 1
X: PEI activation time 3.4611 0.42836 8.08 < 0.0001* 1
Y: GA % 4.7944 0.42836 11.19 < 0.0001* 1
Z: GA activation time − 2.05 0.42836 − 4.79 0.0007* 1
WX − 0.5562 0.45435 − 1.22 0.2489 1
WY 2.2437 0.45435 4.94 0.0006* 1
WZ − 1.5187 0.45435 − 3.34 0.0075* 1
XY − 0.1312 0.45435 − 0.29 0.7786 1
XZ − 0.8187 0.45435 − 1.80 0.1017 1
YZ − 1.8188 0.45435 − 4.00 0.0025* 1
W2 2.4867 1.13883 2.18 0.0539 1.9789
X2 − 7.4132 1.13883 − 6.51 < 0.0001* 1.9789
Y2 2.3867 1.13883 2.10 0.0625 1.9789
Z2 7.8867 1.13883 6.93 < 0.0001* 1.9789