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Table 3 Statistical analysis of CCD showing coefficients, t- values, P-values and standard errors

From: Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application

Term

Coefficient estimate

Std Error

t ratio

P value Prob > F

VIF

Intercept

57.3542

0.78473

73.09

< 0.0001*

1

W: PEI %

1.7389

0.42836

4.06

0.0023*

1

X: PEI activation time

3.4611

0.42836

8.08

< 0.0001*

1

Y: GA %

4.7944

0.42836

11.19

< 0.0001*

1

Z: GA activation time

− 2.05

0.42836

− 4.79

0.0007*

1

WX

− 0.5562

0.45435

− 1.22

0.2489

1

WY

2.2437

0.45435

4.94

0.0006*

1

WZ

− 1.5187

0.45435

− 3.34

0.0075*

1

XY

− 0.1312

0.45435

− 0.29

0.7786

1

XZ

− 0.8187

0.45435

− 1.80

0.1017

1

YZ

− 1.8188

0.45435

− 4.00

0.0025*

1

W2

2.4867

1.13883

2.18

0.0539

1.9789

X2

− 7.4132

1.13883

− 6.51

< 0.0001*

1.9789

Y2

2.3867

1.13883

2.10

0.0625

1.9789

Z2

7.8867

1.13883

6.93

< 0.0001*

1.9789