TY - JOUR AU - Abdella, Mohamed A. A. AU - El-Sherbiny, Gamal M. AU - El-Shamy, Aliaa R. AU - Atalla, Sherien M. M. AU - Ahmed, Samia A. PY - 2020 DA - 2020/03/20 TI - Statistical optimization of chemical modification of chitosan-magnetic nano-particles beads to promote Bacillus subtilis MK1 α-amylase immobilization and its application JO - Bulletin of the National Research Centre SP - 40 VL - 44 IS - 1 AB - α-Amylase randomly hydrolyzes starch molecule to produce oligosaccharides of different chain length. It is among the most significant hydrolytic enzymes used in industrial applications. Enzyme immobilization is the simplest way to solve the stability problem of protein under industrial harsh conditions. Magnetic nano-particles considered suitable for immobilization due to their unique characteristics. The polymer nanocarriers still the feature of modifiable surfaces of carriers for further conjugation with biomolecule. This study aims to promote the immobilization of Bacillus subtilis MK1 α-amylase using the statistical optimization of the chemical modification of the chitosan-magnetic nano-magnetic particle beads and their ability to apply. SN - 2522-8307 UR - https://doi.org/10.1186/s42269-020-00301-3 DO - 10.1186/s42269-020-00301-3 ID - Abdella2020 ER -