Enhancement effects of ethanolic extract of Fagonia cretica on Bisphenol A (BPA)-induced genotoxicity and biochemical changes in rats

Bulletin of the National Research Centre

Table 1 Comet assay analysis shows the enhancement role of Fagonia cretica (FCE) on Bisphenol A-induced DNA damage in rats

Treatment	No of animals	No. of analyzes cells*	Comets	Comet classes^**				DNA damaged cells % (mean ± SEM)
Treatment	No of animals	No. of analyzes cells*	Comets	0	1	2	3	DNA damaged cells % (mean ± SEM)
Normal control	5	500	31	469	19	12	0	6.2 ± 1.30^c
Control oil treatment	5	500	34	466	21	13	0	6.8 ± 1.30^c
Bisphenol A (BPA)	5	500	139	361	42	53	44	27.8 ± 2.59^a
Protective low dose of FCE	5	500	124	376	36	42	46	24.8 ± 0.84^a
Protective medium dose of FCE	5	500	86	414	31	26	29	17.2 ± 1.92^b
Protective high dose of FCE	5	500	77	423	28	27	22	15.4 ± 2.61^b
Therapeutic low dose of FCE	5	500	126	374	39	45	42	25.2 ± 1.92^a
Therapeutic medium dose of FCE	5	500	113	387	34	44	35	22.6 ± 1.52^ab
Therapeutic high dose of FCE	5	500	106	394	31	38	37	21.2 ± 1.64^ab

*Number of cells examined per a group, ^**Class 0 = no tail; Class1 = tail length < diameter of the nucleus; Class 2 = tail length between 1× and 2× the diameter of the nucleus; and Class 3 = tail length > 2× the diameter of the nucleus
All data are expressed as mean ± SE. Means with different superscripts letters a, b, c are significantly different at P ≤ 0.05