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Fig. 6 | Bulletin of the National Research Centre

Fig. 6

From: GAPDH spike RNA as an alternative for housekeeping genes in relative gene expression assay using real-time PCR

Fig. 6

ISG56 expression EV control cells. Cells were plated at a density of 1 × 105/ml for 24 h. HSP70 was depleted transiently from EV control cells (Atwan, 2016). RNA samples were extracted and spiked either directly and before DNase treatment or spiked or after DNase treatment before reverse transcription or spike RNA was added to the well of qPCR reaction after reverse transcribed separately. Total ISG56 RNA was quantified by RT-qPCR using 10 ng/well of its (cDNA) as a template. Data were calculated by the ΔΔCt method, using GAPDH as internal control, and then normalized to the control cell value which was set to be 1. a Normalized to GAPDHmRNA. b Normalized to spike GAPDH mRNA

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