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Bulletin of the National Research Centre
Fig. 5 | Bulletin of the National Research Centre

Fig. 5

From: GAPDH spike RNA as an alternative for housekeeping genes in relative gene expression assay using real-time PCR

Fig. 5

PML-II expression in PML-II depleted and EV control cells. Cells were plated at a density of 1 × 105/ml for 24 h. RNA samples were extracted either directly or spiked with 1 ng of GAPDH RNA or left as a regular extracted RBA. Total PML-II RNA was quantified by RT-qPCR using 10 ng/well of its (cDNA) as a template. Data were calculated by the ΔΔCt method, using GAPDH as internal control, and then normalized to the control cell value which was set to be 1. a Normalized to GAPDH mRNA. b Normalized to spike GAPDH mRNA

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