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Bulletin of the National Research Centre
Fig. 3 | Bulletin of the National Research Centre

Fig. 3

From: GAPDH spike RNA as an alternative for housekeeping genes in relative gene expression assay using real-time PCR

Fig. 3

Hexon expression in GFP control cell lines normalized to GAPDH at 37 °C and 40 °C. GFP cells were plated at a density of 1 × 105/ml for 24 h. Cells were infected for 20 h at moi of 5 with human adenovirus type 5, RNA samples were extracted, and total hexon RNA was quantified by RT-qPCR using 10 ng/well of its (cDNA) as a template. Data were calculated by the ΔΔCt method, using GAPDH as internal control, and then normalized to the control cell value which was set to be 1

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