Fig. 7From: Effect of etoposide on grass pea DNA topoisomerase II: an in silico, in vivo, and in vitro assessmentsPlasmid linearize assay by purified plant topoII with increasing concentration of etoposide. C1, pBS plasmid; C2, plasmid and etoposide; Lanes 3 to 7, plasmid DNA incubated with purified plant topoII and etoposide (0, 10, 20, 30, and 40 μM); C3, EcoR1 digested plasmid. With the increasing concentrations of etoposide (≥ 20 μM) smearing of DNA are appeared due to the cleavage of DNA at multiple sitesBack to article page