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Table 5 Number of new appeared (+) and disappeared (−) bands as compared to control and genomic template stability (GTS) percentage of moringa seedlings pre-treated with laser light He–Ne using twelve ISSR primers

From: The influence of He-Ne laser on agro-morphological criteria, ISSR marker and SDS-PAGE of Moringa oleifera

PrimerDry seedsWetted seedsCont
25 min45 min90 min25 min45 min90 min
ISSR-6+4911107, 2661579, 266, 2321107, 26665765724
0657, 257257546, 491, 417, 2571579, 546, 4171579, 600, 546, 491
ISSR-7+0509, 2020044667321
2042041413, 473, 431, 2045091413, 998, 473, 431, 509, 202509, 202
ISSR-8+0398000012
00000398
ISSR-9+366366366463507, 46336617
46305076120612
ISSR-10+0000251010
000000
ISSR-11+0001264, 4280013
867, 4281264, 8671264, 867000
ISSR-12+390, 3231341, 7443231491, 1251, 744, 390, 3541341, 744, 684, 496, 402, 345, 2541491, 684, 40217
402, 3451251, 1972, 6841251, 818, 402, 345, 3078180307
ISSR-13+1333, 177573, 251329, 177251, 310, 1641158, 285, 1521185, 801, 251, 15221
1158, 916, 8011435, 1158, 916, 827, 801, 471, 1521435, 916, 827, 471329, 177280177
ISSR-16+987, 675987, 67501618, 1270, 900, 479, 4381270, 900, 479, 4381270, 900, 479, 43811
1270, 703, 607703, 6071270, 807000
ISSR-HB9+0674, 653, 504674, 653, 504, 201504007
380000653674, 653, 380, 300
ISSR-HB13+524, 476, 418632, 440, 349, 288440, 321733, 632, 476, 440, 349, 321, 288632, 288871, 733, 476, 32112
871, 733, 595871, 595871, 733595, 398595595
ISSR-HB14+0001189, 4234234237
423423423109210921092
Total number of bands283935403535172
GTS%83.72%77.32%79.65%76.74%79.65%79.65%100%
  1. (+) appearance of new bands, (−) disappearance of normal bands