Table 5 Number of new appeared (+) and disappeared (−) bands as compared to control and genomic template stability (GTS) percentage of moringa seedlings pre-treated with laser light He–Ne using twelve ISSR primers
Primer | Dry seeds | Wetted seeds | Cont | |||||
|---|---|---|---|---|---|---|---|---|
25 min | 45 min | 90 min | 25 min | 45 min | 90 min | |||
ISSR-6 | + | 491 | 1107, 266 | 1579, 266, 232 | 1107, 266 | 657 | 657 | 24 |
− | 0 | 657, 257 | 257 | 546, 491, 417, 257 | 1579, 546, 417 | 1579, 600, 546, 491 | ||
ISSR-7 | + | 0 | 509, 202 | 0 | 0 | 446 | 673 | 21 |
− | 204 | 204 | 1413, 473, 431, 204 | 509 | 1413, 998, 473, 431, 509, 202 | 509, 202 | ||
ISSR-8 | + | 0 | 398 | 0 | 0 | 0 | 0 | 12 |
− | 0 | 0 | 0 | 0 | 0 | 398 | ||
ISSR-9 | + | 366 | 366 | 366 | 463 | 507, 463 | 366 | 17 |
− | 463 | 0 | 507 | 612 | 0 | 612 | ||
ISSR-10 | + | 0 | 0 | 0 | 0 | 251 | 0 | 10 |
− | 0 | 0 | 0 | 0 | 0 | 0 | ||
ISSR-11 | + | 0 | 0 | 0 | 1264, 428 | 0 | 0 | 13 |
− | 867, 428 | 1264, 867 | 1264, 867 | 0 | 0 | 0 | ||
ISSR-12 | + | 390, 323 | 1341, 744 | 323 | 1491, 1251, 744, 390, 354 | 1341, 744, 684, 496, 402, 345, 254 | 1491, 684, 402 | 17 |
− | 402, 345 | 1251, 1972, 684 | 1251, 818, 402, 345, 307 | 818 | 0 | 307 | ||
ISSR-13 | + | 1333, 177 | 573, 251 | 329, 177 | 251, 310, 164 | 1158, 285, 152 | 1185, 801, 251, 152 | 21 |
− | 1158, 916, 801 | 1435, 1158, 916, 827, 801, 471, 152 | 1435, 916, 827, 471 | 329, 177 | 280 | 177 | ||
ISSR-16 | + | 987, 675 | 987, 675 | 0 | 1618, 1270, 900, 479, 438 | 1270, 900, 479, 438 | 1270, 900, 479, 438 | 11 |
− | 1270, 703, 607 | 703, 607 | 1270, 807 | 0 | 0 | 0 | ||
ISSR-HB9 | + | 0 | 674, 653, 504 | 674, 653, 504, 201 | 504 | 0 | 0 | 7 |
− | 380 | 0 | 0 | 0 | 653 | 674, 653, 380, 300 | ||
ISSR-HB13 | + | 524, 476, 418 | 632, 440, 349, 288 | 440, 321 | 733, 632, 476, 440, 349, 321, 288 | 632, 288 | 871, 733, 476, 321 | 12 |
− | 871, 733, 595 | 871, 595 | 871, 733 | 595, 398 | 595 | 595 | ||
ISSR-HB14 | + | 0 | 0 | 0 | 1189, 423 | 423 | 423 | 7 |
− | 423 | 423 | 423 | 1092 | 1092 | 1092 | ||
Total number of bands | 28 | 39 | 35 | 40 | 35 | 35 | 172 | |
GTS% | 83.72% | 77.32% | 79.65% | 76.74% | 79.65% | 79.65% | 100% | |