Table 2 General features of SL1 and SL3 metagenomes
From: Mercury contamination imposes structural shift on the microbial community of an agricultural soil
| Â | SL1 | SL3 |
|---|---|---|
1.Pre-processing | ||
 a. Raw reads |  |  |
  Reads | 46,292 | 27,220 |
  Total bases (bp) | 13,787,457 | 7,604,708 |
  Mean read length (bp) | 297.84 ± 27.40 | 279.38 ± 72.10 |
  Mean GC content (%) | 54.77 ± 7.14 | 52.82 ± 16.09 |
 b. Quality trimming | ||
  Trimmed reads | ||
  Reads | 45,795 (98.93%) | 25,075 (92.12%) |
  Total bases (bp) | 13,769,735 (99.87%) | 7,529,285 (99.01%) |
  Mean read length (bp) | 300.68 ± 1.78 | 300.27 ± 10.25 |
  Mean GC content (%) | 55.33 ± 4.48 | 57.23 ± 5.34 |
  Paired reads | 45,784 (99.98 %) | 24,976 (99.61 %) |
  Paired total bases | 13,766,902 (99.98 %) | 7,510,110 (99.75 %) |
  Unpaired reads | 11 (0.02 %) | 99 (0.39 %) |
  Unpaired total bases | 2,833 (0.02 %) | 19,175 (0.25 %) |
2 Assembly and annotation | ||
 a. De novo assembly by idba_ud | ||
  Number of contigs | 68 | 38 |
  N50 (bp) | 423 | 424 |
  Max contig size (bp) | 465 | 458 |
  Min contig size (bp) | 326 | 262 |
  Total assembly size (bp) | 27,929 | 15,691 |
 b. Assembly validation by read mapping | ||
  Number of mapped reads | 31,315 | 15,774 |
  % of Total reads | 68.38% | 62.91% |
  Number of unmapped reads | 14,480 | 9,301 |
  % of Total reads | 31.62 % | 37.09 % |
  Average fold coverage | 276.60 X | 275.14 X |