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Table 7 Phenolic compound determination in gardenia calli cultures cultured on MS media contained different concentrations of phenyl alanine or salicylic acid during the third harvested period of culturing by HPLC

From: Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid

Treatment Phenolic compounds determined in gardenia callus cultures in μg/g dry weight in the third harvest t. P
  Cinnamic Rosmarinic P-hydroxy Protocatechuic Gallic Catechin Caffeic Syringic p-coumaric Vanillic Sinapic
Control 79.95 ND N.D. 128.177 85.13 N.D. N.D. N.D. N.D. N.D. N.D. 293.25
2 mg/l phenylalanine 49.87 213.16 18.76 14.2414 ND 297.23 7.14 30.71 43.47 4.86 9.0074 688.44
3 mg/l phenylalanine 92.24 ND ND ND ND ND ND ND 5.21 ND ND 97.45
4 mg/l phenylalanine 49.36 40.26 19.13 ND ND 54.72 ND 9.51 1.97 ND ND 174.95
5 mg/l phenylalanine 68.997 15.52 10.32 ND ND 40.28 ND ND 5.094 ND ND 140.21
0.25 mM salicylic acid (34.5 mg/l) 52.48 19.72 ND ND ND 138.59 5.35 105.9 14.87 68.23 ND 405.14
0.5 mM salicylic acid (69 mg/l) 72.37 22.48 5.21 ND ND 180.80 ND 2.35 20.975 ND 5.4139 309.59
0.75 mM salicylic acid (103.5 mg/l) ND 24.06 45.46 ND ND 246.65 ND ND 41.45 ND 8.24 365.86
1 mM salicylic acid (138 mg/l) 49.65 78.20 11.40 ND ND 493.65 5.105 3.07 2.55 ND 6.12 649.74
  1. ND not detected