Table 7 Phenolic compound determination in gardenia calli cultures cultured on MS media contained different concentrations of phenyl alanine or salicylic acid during the third harvested period of culturing by HPLC
From: Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid
Treatment | Phenolic compounds determined in gardenia callus cultures in μg/g dry weight in the third harvest | t. P | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cinnamic | Rosmarinic | P-hydroxy | Protocatechuic | Gallic | Catechin | Caffeic | Syringic | p-coumaric | Vanillic | Sinapic | ||
Control | 79.95 | ND | N.D. | 128.177 | 85.13 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 293.25 |
2 mg/l phenylalanine | 49.87 | 213.16 | 18.76 | 14.2414 | ND | 297.23 | 7.14 | 30.71 | 43.47 | 4.86 | 9.0074 | 688.44 |
3 mg/l phenylalanine | 92.24 | ND | ND | ND | ND | ND | ND | ND | 5.21 | ND | ND | 97.45 |
4 mg/l phenylalanine | 49.36 | 40.26 | 19.13 | ND | ND | 54.72 | ND | 9.51 | 1.97 | ND | ND | 174.95 |
5 mg/l phenylalanine | 68.997 | 15.52 | 10.32 | ND | ND | 40.28 | ND | ND | 5.094 | ND | ND | 140.21 |
0.25 mM salicylic acid (34.5 mg/l) | 52.48 | 19.72 | ND | ND | ND | 138.59 | 5.35 | 105.9 | 14.87 | 68.23 | ND | 405.14 |
0.5 mM salicylic acid (69 mg/l) | 72.37 | 22.48 | 5.21 | ND | ND | 180.80 | ND | 2.35 | 20.975 | ND | 5.4139 | 309.59 |
0.75 mM salicylic acid (103.5 mg/l) | ND | 24.06 | 45.46 | ND | ND | 246.65 | ND | ND | 41.45 | ND | 8.24 | 365.86 |
1 mM salicylic acid (138 mg/l) | 49.65 | 78.20 | 11.40 | ND | ND | 493.65 | 5.105 | 3.07 | 2.55 | ND | 6.12 | 649.74 |