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Table 4 Flavonoids determination in gardenia calli cultures cultured on MS media contained different concentrations of phenyl alanine or salicylic acid during the three harvested periods of culturing by HPLC

From: Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid

Treatments Flavonoids determined in gardenia callus cultures in μg/g dry weight
First harvest (after 2 weeks of culturing) Second harvest (after 4 weeks of culturing) Third harvest (after 5 weeks of culturing)
Rutin App. Kaemp. Gent. t. F Ruin App. Kaemp. Gent. t.f. Rutin App. Kaemp. Gent. t.f..
Control 22.11 ND ND ND. 22.11 ND ND ND ND ND 20.75 ND ND ND 20.75
2 mg/l phenylalanine 111.3 ND ND ND 111.3 77.56 ND ND ND 77.56 429.8 213.16 2.993 ND 645.95
3 mg/l phenylalanine 201.0 ND ND ND 201.0 152.0 23.22 ND ND 175.22 8.178 8.41 1.99 ND 18.57
4 mg/l phenylalanine 20.31 ND ND ND 20.31 50.49 5.68 ND ND 56.17 137.9 31.09 4.32 ND 173.31
5 mg/l phenylalanine 20.76 ND ND ND 20.76 119.57 ND 7.18 ND 126.75 55.91 13.73 1.66 ND 71.3
0.25 mM salicylic acid(34.5 mg/l) 96.44 ND ND ND 96.44 40.82 ND 4.90 ND 45.72 7.05 19.52 12.47 ND 39.04
0.5 mM salicylic acid(69mg/l) 202.2 ND ND ND 202.2 30.75 5.97 ND ND 36.72 62.78 4.00 6.837 ND 73.61
0.75 mM salicylic acid(103.5mg/l) 14.06 ND ND ND 14.06 24.93 ND ND ND 24.93 75.24 N.D 3.061 3.95 82.25
1 mM salicylic acid(138mg/l) 153.4 ND ND ND 153.4 ND ND ND ND ND 148.2 13.47 1.88 10.36 173.91
  1. ND not detected