Table 3 Number of amplification and polymorphic products using nine primers in rice cultivars and their somaclons
Primer number | Nucleotide sequence(5′–3′) | No. of amplification (A) | No. of polymorphic (B) | Polymorphism b/a (%) | PIC |
|---|---|---|---|---|---|
1 | GAAACGGGTG | 7 | 7 | 100 | 0.851 |
2 | GGGTAACGCC | 8 | 8 | 100 | 0.872 |
3 | CAATCGCCGT | 9 | 6 | 66.7 | 0.878 |
4 | TCGGCGATAG | 10 | 7 | 70 | 0.889 |
5 | TTCCGAACCC | 4 | 3 | 75 | 0.715 |
6 | AGCCAGCGAA | 8 | 7 | 87.5 | 0.862 |
7 | GACCGCTTGT | 9 | 9 | 100 | 0.882 |
8 | AGGTGACCGT | 12 | 11 | 91.7 | 0.906 |
9 | CAAACGTCGG | 10 | 10 | 100 | 0.886 |
Total | 77 | 68 | – | – | |
Mean | 8.5 | 7.5 | 87.88 | 8.6 | |
Maximal | 12 | 11 | 100 | 0.906 | |
Minimal | 4 | 3 | 70 | 0.715 |