Sr. No. | Primer | Sequence (5′→3′) | TN | Tm (°C) | PB | MB | TB | % P | PIC | AS (bp) | Unique band |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | ISSR 03 | (CA)9GG | 20 | 59.4 | 13 | 00 | 13 | 100.0 | 0.888 | 304 to 2310 | 530 bp in IC-381135 |
2 | ISSR 05 | (CA)9TT | 20 | 55.3 | 5 | 00 | 05 | 100.0 | 0.683 | 203 to 1513 | 320 bp in Deesa local |
3 | ISSR 06 | (CA)9TC | 20 | 57.3 | 10 | 01 | 11 | 90.91 | 0.881 | 367 to 2618 | 3140 bp in Suvarna |
4 | ISSR 09 | (CA)9CT | 20 | 57.3 | 09 | 03 | 12 | 75.00 | 0.882 | 236 to 2222 | 995 bp in A. edulis |
5 | ISSR 10 | (CA)9CC | 20 | 59.4 | 09 | 02 | 11 | 81.82 | 0.878 | 200 to 1605 | 900 bp in A. edulis |
6 | ISSR 15 | (CA)9AG | 20 | 57.3 | 09 | 01 | 10 | 90.00 | 0.892 | 257 to 1278 | – |
7 | ISSR 16 | (CA)9AA | 20 | 55.3 | 10 | 01 | 11 | 90.91 | 0.866 | 346 to 2240 | 2232 bp in VL-44 |
8 | ISSR 21 | GC (CT)8 | 18 | 56.0 | 08 | 02 | 10 | 80.00 | 0.831 | 217 to 3189 | 1573 bp in Suvarna; 190 bp in VL-44 |
9 | ISSR 22 | GA (CT)8 | 18 | 53.7 | 07 | 00 | 07 | 100.0 | 0.806 | 201 to 1335 | – |
10 | ISSR 25 | GCC (CT)8 | 19 | 58.8 | 09 | 03 | 12 | 75.00 | 0.893 | 275 to 3702 | – |
11 | ISSR 26 | GCA (CT)8 | 19 | 56.7 | 09 | 03 | 12 | 75.00 | 0.881 | 325 to 3561 | 3561 bp in EC-519549 |
Total | – | – | – | 98 | 16 | 114 | – | – | – | – | |
Mean | – | – | – | 8.91 | 1.45 | 10.36 | 87.15 | 0.853 | – | – | |
Range | – | – | – | – | – | – | 75.00–100.00 | 0.683–0.893 | 200–3702 | – | |
TN number of nucleotide, Tm melting temperature, PB number of polymorphic bands, MB number of monomorphic bands, TB number of total bands, %P percent polymorphism, AS amplicon size, bp base pairs |