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Table 2 Particulars of polymorphic ISSR primers used in the study

From: Genetic diversity analysis and molecular characterization of grain amaranth genotypes using inter simple sequence repeat (ISSR) markers

Sr. No.

Primer

Sequence (5′→3′)

TN

Tm (°C)

PB

MB

TB

% P

PIC

AS (bp)

Unique band

1

ISSR 03

(CA)9GG

20

59.4

13

00

13

100.0

0.888

304 to 2310

530 bp in IC-381135

2

ISSR 05

(CA)9TT

20

55.3

5

00

05

100.0

0.683

203 to 1513

320 bp in Deesa local

3

ISSR 06

(CA)9TC

20

57.3

10

01

11

90.91

0.881

367 to 2618

3140 bp in Suvarna

4

ISSR 09

(CA)9CT

20

57.3

09

03

12

75.00

0.882

236 to 2222

995 bp in A. edulis

5

ISSR 10

(CA)9CC

20

59.4

09

02

11

81.82

0.878

200 to 1605

900 bp in A. edulis

6

ISSR 15

(CA)9AG

20

57.3

09

01

10

90.00

0.892

257 to 1278

7

ISSR 16

(CA)9AA

20

55.3

10

01

11

90.91

0.866

346 to 2240

2232 bp in VL-44

8

ISSR 21

GC (CT)8

18

56.0

08

02

10

80.00

0.831

217 to 3189

1573 bp in Suvarna; 190 bp in VL-44

9

ISSR 22

GA (CT)8

18

53.7

07

00

07

100.0

0.806

201 to 1335

10

ISSR 25

GCC (CT)8

19

58.8

09

03

12

75.00

0.893

275 to 3702

11

ISSR 26

GCA (CT)8

19

56.7

09

03

12

75.00

0.881

325 to 3561

3561 bp in EC-519549

Total

98

16

114

Mean

8.91

1.45

10.36

87.15

0.853

Range

75.00–100.00

0.683–0.893

200–3702

TN number of nucleotide, Tm melting temperature, PB number of polymorphic bands, MB number of monomorphic bands, TB number of total bands, %P percent polymorphism, AS amplicon size, bp base pairs