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Table 2 Particulars of polymorphic ISSR primers used in the study

From: Genetic diversity analysis and molecular characterization of grain amaranth genotypes using inter simple sequence repeat (ISSR) markers

Sr. No. Primer Sequence (5′→3′) TN Tm (°C) PB MB TB % P PIC AS (bp) Unique band
1 ISSR 03 (CA)9GG 20 59.4 13 00 13 100.0 0.888 304 to 2310 530 bp in IC-381135
2 ISSR 05 (CA)9TT 20 55.3 5 00 05 100.0 0.683 203 to 1513 320 bp in Deesa local
3 ISSR 06 (CA)9TC 20 57.3 10 01 11 90.91 0.881 367 to 2618 3140 bp in Suvarna
4 ISSR 09 (CA)9CT 20 57.3 09 03 12 75.00 0.882 236 to 2222 995 bp in A. edulis
5 ISSR 10 (CA)9CC 20 59.4 09 02 11 81.82 0.878 200 to 1605 900 bp in A. edulis
6 ISSR 15 (CA)9AG 20 57.3 09 01 10 90.00 0.892 257 to 1278
7 ISSR 16 (CA)9AA 20 55.3 10 01 11 90.91 0.866 346 to 2240 2232 bp in VL-44
8 ISSR 21 GC (CT)8 18 56.0 08 02 10 80.00 0.831 217 to 3189 1573 bp in Suvarna; 190 bp in VL-44
9 ISSR 22 GA (CT)8 18 53.7 07 00 07 100.0 0.806 201 to 1335
10 ISSR 25 GCC (CT)8 19 58.8 09 03 12 75.00 0.893 275 to 3702
11 ISSR 26 GCA (CT)8 19 56.7 09 03 12 75.00 0.881 325 to 3561 3561 bp in EC-519549
Total 98 16 114
Mean 8.91 1.45 10.36 87.15 0.853
Range 75.00–100.00 0.683–0.893 200–3702
TN number of nucleotide, Tm melting temperature, PB number of polymorphic bands, MB number of monomorphic bands, TB number of total bands, %P percent polymorphism, AS amplicon size, bp base pairs