Table 3 CRISPR-CAS9 constructs to knockout TrxG and PcG genes+
From: Generation of CRISPR-cas9 construct for knockout of genes encoding chromatin-associated proteins
S. no | Name of the target gene | No. of guides | Name of guides | Conformation by PCR | Conformation by sequencing |
|---|---|---|---|---|---|
1 | ISWI | 03 | ISWI21, | YES | YES |
22, | YES | YES | |||
23 | YES | YES | |||
2 | Acf | 02 | Acf 10 | YES | YES |
Acf11 | YES | YES | |||
3 | Pc | 02 | Pc2 | YES | YES |
4 | Psc | 02 | Psc8 | YES | YES |
Psc9 | YES | YES |