Table 1 Guide sequences of genes under study
From: Generation of CRISPR-cas9 construct for knockout of genes encoding chromatin-associated proteins
S. no. | Name of the target gene | No. of guides | Name of guides | Guide sequence 5′ to 3’ |
|---|---|---|---|---|
01. | ISWI | 03 | ISWI19, ISWI20, ISWI21, ISWI22 and ISWI23 | TTCGACGGCAGCTGTATCTGTT, TTCGGGTCTACCCTTAGGCTTCGT, TTCGATATCTCCGTCTGCTTTAGC, TTCGCTTTTCACCGGATGAACTGG, TTCGTAAGAGTCCCACGAAGCCTA |
02. | Acf | 02 | Acf10 and Acf11 | TTCGCCTTCGGGCACCCGTCCTTC, TTCGGGTGTTGACCGCCGACTGTT |
03. | Pc | 02 | Pc1, Pc2 | TTCGGCCGCGTACACTAGATCGAC, TTCGTCCAGTCGATCTAGTGTACG |
04. | Psc | 02 | Psc8 and Psc9 | TTCGGGCTGTATTGCTTTCGATTC, TTCGGACAACGGCAACGAATAAGG |