Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa

Bulletin of the National Research Centre

Table 1 Effect of the EH_GH–pmKate2–N vector uptake on sperm motility (mean ± SEM)

Parameters	*Negative control	EH_GH–pmKate2–N expression vector groups
Parameters	*Negative control	**Positive control	^‡‡DMSO
Total motility (%)	64.0 ± 0.8^b	64.7 ± 0.6^b	66.5 ± 0.6^a
Progressive motility (%)	51.8 ± 0.8^b	48.4 ± 0.8^c	52.7 ± 0.7^a
DAP (μm)	23.8 ± 0.3^a	23.1 ± 0.3^a,b	22.9 ± 0.3^b
DCL (μm)	38.0 ± 0.4^a	36.5 ± 0.5^b	37.4 ± 0.4^a,b
DSL (μm)	20.6 ± 0.3^a	20.0 ± 0.4^a,b	19.6 ± 0.2^b
VAP (μm/s)	51.8 ± 0.6^a	50.0 ± 0.8^b	49.6 ± 0.6^b
VCL (μm/s)	82.6 ± 0.9^a	78.9 ± 1.0^c	81.0 ± 0.6^b
VSL (μm/s)	44.7c ± 0.6^a	43.1 ± 0.8^b	42.5 ± 0.5^b
STR (VSL/VAP, %)	0.86 ± 0.00^a	0.85 ± 0.0^b	0.84 ± 0.00^c
LIN (VSL/CCL, %)	0.54 ± 0.00 ^a	0.54 ± 0.0^a	0.52 ± 0.00^b
WOBa (VAP/VCL)	0.62 ± 0.00^a	0.62 ± 0.0^a	0.61 ± 0.00^b
ALH (μm)	2.9 ± 0.04^a	2.8 ± 0.03^b	2.8 ± 0.02^b
BCF (H2)	28.8 ± 0.2^a	27.0 ± 0.28^b	28.5 ± 0.24^a

Mean values with different superscript letters within the same row differ (P < 0.05). *Negative control, sperm cells incubated at 37 °C for 1 h without vector. **Positive control, sperm cells incubated at 37 °C for 1 h with vector. ^‡‡DMSO group, sperm cells incubated with vector and submitted to 3% DMSO then incubated at 37 °C for 1 h. DAP distance average path (microns), DCL distance curved line (microns), DSL distance straight line (microns), VAP velocity average line (microns/s), VCL velocity curved line (microns/s), VSL velocity straight line (microns/s), STR straightness (VSL/VAP), LIN linearity (VSL/VCL), WOB wobble—describes side to side movement of the sperm head (VAP/VCL), ALH amplitude of lateral head displacement (microns), BCF beat cross frequency (H2)