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Fig. 1 | Bulletin of the National Research Centre

Fig. 1

From: Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa

Fig. 1

The backbone of the pmKate2–N expression vector. MCS, multiple cloning site for cloned insertion; mKate2, far-red fluorescent protein; PCMV IE, immediate early promoter of cytomegalovirus; SV40 ori, origin for replication in mammalian cells; pUC ori, origin of replication for propagation in E. coli; f1 ori, origin for single-stranded DNA production; SV40 poly A, polyadenylation signals; PSV40, early promoter provides neomycin resistance gene (Neor) expression to select stably transfected eukaryotic cells using G418; P, bacterial promoter provides kanamycin resistance gene expression (Kanr) in E. coli; Kanr/Neor, gene is linked with herpes simplex virus (HSV) thymidine kinase (TK) polyadenylation signals (Shcherbo et al. 2009)

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