Table 7 Primer developed for the selected species
From: Development of DNA-based species identification and barcoding of three important timbers
Species | Barcode loci | Primer information | Size (bp) | Product size (bp) | Primer temperature (°C) |
|---|---|---|---|---|---|
T. grandis | ITS | F′ CGATATCTTGGCAGCATTCC R′ TTGCGTTCAAAGACTCGATG | 20/20 | 232 | 61.0 |
rbcL | F′ GACAACTGTGTGGACCGATG R′ AGAGCACGTAGGGCTTTGAA | 20/20 | 201 | 60.0 | |
D. latifolia | ITS | F′ AGGAAGCAACAACCGAACAG R′ TTGCGTTCAAAGACTCGATG | 20/20 | 192 | 60.0 |
rbcL | F′GCTGCCGAATCTTCTACTGG R′ GGATTCGCAAATCTTCCAGA | 20/20 | 250 | 60.0 | |
L. lanceolata | ITS | F′ GCTCCATCGCTCGTTTTTAG R′ CCGTGAACCATCGAGTCTTT | 20/20 | 210 | 60.0 |
rbcL | F′ CAGGGCTTTGAACCCAAATA R′ GACAACTGTGTGGACCGATG | 20/20 | 193 | 60.0 |