Protective effect of Bacillus subtilis, B. pumilus, and Pseudomonas fluorescens isolates against root knot nematode Meloidogyne incognita on cowpea

Bulletin of the National Research Centre

Table 1 Effects of Bacillus subtilis, B. pumilus, and Pseudomonas fluorescens isolates on Meloidogyne incognita reproductive parameters on cowpea grown under screen house conditions

Treatments	M. incognita nematode parameters								Average total percentage nematode parameter reduction (%)
	J₂s in soil/pot		J₂s in roots/plant		Galls/plant		Eggmasses/plant
	No.	Red. (%)	No.	Red. (%)	No.	Red (%)	No.	Red. (%)
B. subtilis (Bs₁)	4270ef	86	223bcd	69	24def	73	19de	74	75.5
B. subtilis (Bs₂)	3050f	75	113de	84	16f	82	10f	87	82.0
B. pumilus (Bp₁)	7600de	88	260bc	64	27cde	70	21cde	72	73.5
B. pumilus (Bp₂)	3670de	90	155cde	79	32bcd	64	25bcd	66	81.8
P. fluorescens (Pf₁)	12,400c	60	210bcd	71	23ef	74	19de	74	69.8
P. fluorescens (Pf₂)	7370de	76	323b	56	38b	57	30b	60	62.3
Carbofuran 10%	9530cd	69	77e	89	26de	71	17ef	77	76.5
Medium only	17,000b	55	247bc	66	35bc	61	27bc	64	61.5
Nematode only (control)	30,800a	–	728a	–	89a	–	74a	–	–

Means are averages of four replicates. Means followed by different letter(s) are significantly different according to Duncan^’s multiple range test at P ≤ 0.05. Red. reduction