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Table 5 Effects on peroxidase, polyphenoloxidase, and chitinase enzymes activities of garlic plants under field conditions at 100 days after planting

From: Effect of inoculum density of Stromatinia cepivora on the amount of white rot reduced by Trichoderma species in garlic

Treatment

Enzyme activities in garlic leavesa

Peroxidase

Polyphenoloxidase

Chitinase

Trial I (40 sclerotia/kg soil)

T. harzianum (Th)

0.313 ± 0.005 de

0.435 ± 0.004 d

1.754 ± 0.014 c

T. koningii (Tk)

0.312 ± 0.006 de

0.445 ± 0.004 cd

1.593 ± 0.021 d

T. virens (Tv)

0.331 ± 0.008 cd

0.439 ± 0.003 cd

1.515 ± 0.017 d

(Th) + (Tk)

0.378 ± 0.005 b

0.542 ± 0.009 b

1.909 ± 0.033 b

(Th) + (Tv)

0.379 ± 0.006 b

0.547 ± 0.003 b

1.921 ± 0.036 b

(Tk) + (Tv)

0.339 ± 0.007 c

0.531 ± 0.005 b

1.900 ± 0.025 b

(Th) + (Tk) + (Tv)

0.472 ± 0.004 a

0.587 ± 0.002 a

2.026 ± 0.046 a

Tebuconazole

0.295 ± 0.019 e

0.454 ± 0.014 c

1.386 ± 0.052 e

Control

0.118 ± 0.002 f

0.226 ± 0.004 e

0.763 ± 0.022 f

Trial II (600 sclerotia/kg soil)

T. harzianum (Th)

0.339 ± 0.009 d

0.566 ± 0.011 e

1.806 ± 0.028 d

T. koningii (Tk)

0.372 ± 0.001 c

0.583 ± 0.005 d

1.933 ± 0.012 c

T. virens (Tv)

0.376 ± 0.005 c

0.566 ± 0.005 e

1.918 ± 0.024 c

(Th) + (Tk)

0.419 ± 0.004 b

0.616 ± 0.004 c

2.198 ± 0.018 b

(Th) + (Tv)

0.434 ± 0.013 b

0.632 ± 0.006 b

2.193 ± 0.020 b

(Tk) + (Tv)

0.432 ± 0.003 b

0.635 ± 0.004 b

2.172 ± 0.001 b

(Th) + (Tk) + (Tv)

0.502 ± 0.004 a

0.659 ± 0.005 a

2.692 ± 0.024 a

Tebuconazole

0.329 ± 0.003 d

0.436 ± 0.003 f

1.457 ± 0.064 e

Control

0.139 ± 0.004 e

0.239 ± 0.005 g

0.786 ± 0.017 f

  1. Values are mean of eight replicates for each treatment as well as the control
  2. Means ± standard errors within a column followed by the same letter are not significantly different by Duncan multiple range test at P < 0.05
  3. aPeroxidase activity was expressed as the increase in absorbance at 470 nm/g fresh weight/minute. Polyphenoloxidase activity was expressed as the increase in absorbance at 475 nm/g fresh weight/minute. Chitinase activity was expressed as mM N-acetyl glucose amine equivalent released/gram fresh weight/60 min at 540 nm. Values are means of four replicates