Table 3 Antioxidant activity of jojoba hull extracts at room temperature
Treatment 1 g meal: 30 ml solvent three times, each time ultrasound for 1 h then soaking 24 h | DPPH scavenging effect (% ± SD) | Hydrogen peroxide scavenging effect (% ± SD) | Total reductive capability (m/g) ± SD. |
|---|---|---|---|
Ethanol:1 N HCl 70:30 | 87.6 ± 0.01 | 13.4 ± 0.10 | 15.9 ± 0.01 |
Ethanol:0.5 N HCl 70:30 | 89.4 ± 0.03 | 12.0 ± 0.06 | 17.0 ± 0.02 |
Ethanol:1 N NaOH 70:30 | 8.2 ± 0.02 | 12.6 ± 0.05 | 6.4 ± 0.04 |
Ethanol:0.5 N NaOH 70:30 | 43.6 ± 0.05 | 11.7 ± 0.03 | 9.7 ± 0.03 |
1 N NaOH | 100 ± 0.01 | 100 ± 0.01 | 53.8 ± 0.1 |
0.5 N NaOH | 100 ± 0.01 | 100 ± 0.01 | 40.7 ± 0.07 |
1 N HCl | 93.9 ± 0.04 | 4.9 ± 0.02 | 2.4 ± 0.05 |
0.5 N HCl | 94.8 ± 0.10 | 2.2 ± 0.05 | 1.3 ± 0.01 |
1 N NaOH then neutralize with 1 N HCl | 73.6 ± 0.20 | ND | 72.8 ± 0.20 |
0.5 N NaOH then neutralize with 0.5 N HCl | 77.0 ± 0.20 | ND | 67.4 ± 0.10 |
1 N HCl then neutralize with 1 N NaOH | 87.6 ± 0.01 | ND | 5.7 ± 0.03 |
0.5 N HCl then neutralize with 0.5 N NaOH | 90.0 ± 0.07 | ND | 4.4 2 |
BHT | 51.5 ± 0.3 | 23.7 ± 0.4 | 1.1 ± 0.1 |