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Bulletin of the National Research Centre

Table 3 Amplification products generated with SSR markers among mother plant and in vitro raised plants

From: Analysis of diversity using simple sequence repeat (SSR): distinctions between original Parmentiera cereifera tree and somaclones

Primers

Forward primer (5′ → 3′)

Reverse primer (5′ → 3′)

A

B

C

SSR1

GTGAGCAATTTTGATTATACTG

TACCCTGATGCTGTAATATGTG

2

2

0

SSR2

TCTGGCCAGGATCAATATTACT

TAAGATACATAGATCCAACACC

2

1

1

SSR3

GGTCTTCTGGGCTTTGATCCTG

TGTTGCTAGGGACCCGTAGTGG

2

2

0

SSR4

GAAACAGGGTTAACCATGCCAA

ATGGTGCTGCCAACAACATACA

1

1

0

SSR5

GAGGGTTCTGAAATGTTTTGCC

ACGTGCTAGGGAGGTATCTTGC

3

3

0

SSR6

CTCATGAGTATATCACCGCACA

GACGCGAAACGAATATTCAAGT

2

1

1

SSR7

TTATCTTGGTGTCTCATGTCAG

TCGCAAGATCATCAGAACAGTA

1

1

0

SSR8

TCCAATCAATCAGGGAGGAGTA

GAACGCATCAAGGCATGAAGTA

3

2

1

SSR9

TTCTAAAATGTTTGAAACGCTC

GCATTTCGATATGTTGAAGTAA

2

1

1

SSR10

GTTTTTGTGATCCCGGGTTT

CATGCGTCAGTTCAAGTTTT

2

2

0

SSR11

GTCCATATATGCAAGGAGTC

GTACTCTATCGCAAAACACA

1

1

0

SSR12

AATGTCATGCGTGTAGTAGCCA

AAGCGCACTTAACAGAAGAGGG

1

1

0

SSR13

GCCTCTAGGGAGAAAACTAACA

TCAAGATCATATCCTCCCCAAC

3

3

0

SSR14

ATAAAGCTGTCTCTTTAGTTCG

GTTTTAACACATATGCATACCT

2

2

0

SSR15

AGTGGTAATGAGGTGAAAGAAG

TCGGTCGTATATGCATGTAAAG

2

2

0

SSR16

AACACAAAAGATCCAACGACAC

CAGTATAGAAGGATTTTGAGAG

3

2

1

SSR17

TACCCGAATCTGGAAAATCAAT

TGGAAGCTTGCTAACTTTGGAG

1

1

0

SSR18

TGCAGTTGCGGATCCTTGA

TAACCAAGCAGCACGTATT

1

0

1

SSR19

AGGGCTCTCTTTAATTCTTGCT

GGTCTATCGTAATCCACCTGTA

1

1

0

SSR20

TCCTTGACCCCTTGCACTAACT

ATGGTTGGGAGCACTAGCTTGG

1

0

1

SSR21

GTATCTCACGAGCATAACACAA

GAAAGTGTATGGATCATTAGGC

2

2

0

SSR22

ATTTTCTCAAACACACCCCG

TAGCAGATGTTGACAATGGA

2

1

1

SSR23

ACGTATCCAGACACTGTGGTAA

TAATGGTGGATCCATGATAGCC

1

1

0

SSR24

GAGATTTGTTCATTTCATCTTCGCA

TATATTAAAGGTTAGAGGTAGTCAG

1

1

0

SSR25

GACGTCAAGAATCTTCGTCGGA

ATCTGCTGAGCAGATCGTGGTT

2

2

0

SSR26

GCTCAGATCATCCACCAACTTC

AGATGCTCTGGGAGAGTCCTTA

2

2

0

SSR27

AGTAATCTGGTCCTCTCTTCTTCT

AGGTAATCTCCGAGTGCACTTCAT

1

1

0

SSR28

CCAAATCTTCGAACAAGAACCC

ACCGATCGATGGTGTATACTGA

2

2

0

SSR29

GCTTTAACAAAGATCCAAGTGGCAT

GTAAACATCCAAACAAAGTCGAACG

1

1

0

SSR30

CTCCATCTATTGAGCGAAGGTT

CAAGATGAAGCTCATGCAAGTG

1

1

0

SSR31

GTGGATAACATCATGGTCAAC

TACTTCGCACTAGATGAGCCT

2

2

0

SSR32

TCAGGCCATGTATTATGCAGTA

ACGACCAGGATAGCCAATTCAA

2

2

0

SSR33

AGTTATGTATTCTCTCGAGCCTG

GGTAACCACTAGAGTATGTCCTT

1

1

0

SSR34

GACATGTGCACCAGAATAGC

AGAAGAACTATTCGACTCCT

2

2

0

SSR35

GGAGCATCGCAGGACAGA

GGACGAGGACGCCTGAAT

2

2

0

SSR36

TGCGGTACAGGCAAGGCT

TAGAACGCCCTCGTCGGA

1

0

1

SSR37

ACAAAGGTGCATTCGTAGA

AACACGCATCAGTTTCAGT

1

1

0

SSR38

CCTGTTGCATACTTGACCTTTTT

GGAGTTCAATCTTTCATCACCAT

2

2

0

Total

–

–

64

55

9

Mean

–

–

1.68

1.45

0.236

Maximal

–

–

3

 

2

Minimal

–

–

1

 

0

  1. A = number of scorable bands, B = monomorphic bands, C = polymorphic bands
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