In vitro laser radiation induces mutation and growth in Eustoma grandiflorum plant

Bulletin of the National Research Centre

Table 4 Effect of laser radiation on floral characters of Eustoma grandiflorum adapted plants

Character	Control	Blue (min.)				Green (min.)				Red (min.)
Dose (min.)	(0)	5	10	20	25	5	10	20	25	5	10	20	25
Days to flower bud initiation	177ab	120.33de	134cde	94.67e	103de	133cde	145bcd	122cde	143bcd	198a	164abc	131.33cde	105de
Days to bloom	183.33a	152.67b	144.33b	102d	192.33a	192a	148b	133.5cd	150.33b	203.67a	192a	135bc	113cd
Flowering percentage (%)	18.51c	21.55bc	10.11c	59.25a	9.37c	9.37c	37.03b	37.03b	10.85c	9.37c	9.37c	72.07a	25.92bc
No .of flower buds/plant	2.33cd	7.33b	6bc	12.33a	4.33bcd	2.33cd	5.67bc	5bcd	3.33bcd	1d	3bcd	1.67cd	4bcd
No. of flowers/plant	1.67c	6ab	4bc	8.33a	3.33bc	1c	5.67ab	2.67bc	1.33c	1c	1c	1.67bc	3.67bc
Flower Diameter (cm)	2.33c	2.83bc	2.16c	4.83a	2.16c	2.13c	4.46a	3.8ab	2.86bc	2.13c	2.33c	4.5a	3.83ab
Bloom stem length (cm)	6.5def	8cd	3.5ef	29a	2.5f	2.16f	8.16cd	10.8bc	2.16f	2.3f	2.4f	6.83cde	14b
Peduncle length (cm)	3.67d	6.16c	2.67d	8.5ab	3d	2.83d	6.16c	7.5bc	3.16d	2.9d	2.9d	6.6c	9.7a
Days to flower senescence (from blooming)	3d	10.33ab	4cd	12.67a	3.67cd	3d	9abc	6.67bcd	10ab	3d	3d	7.67abcd	7bcd
No. of petals/flower	11def	14cde	8.33f	20ab	9f	8.33f	17.33bc	24a	9.67f	9f	10ef	15cd	21.67a
Petals area (cm²)	3.76c	1.95cd	1.79d	7.58a	1.98cd	2.04cd	5.72b	5.71b	3.09cd	1.98cd	1.9cd	5.54b	6.44ab
No. of Stamens per flower	4c	5bc	3c	6.67ab	3c	3c	7a	4.67c	4.33c	3c	3.67ab	6.67ab	4.67c
F.W. of flower (g)	0.41bcd	0.24cd	0.16d	1.42a	0.13d	0.15d	0.62bc	0.72b	0.18d	0.031d	0.038d	0.76b	1.33a
D.W. of flower (g)	0.095bcde	0.095bcde	0.058cd	0.14ab	0.44cde	0.025e	0.081bcde	0.12abc	0.051cde	0.015e	0.028de	0.11bcd	0.19a

Means within a column having the same letters are not significantly different according to Duncan’s multiple range test (DMRT) at 5% level