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Fig. 1 | Bulletin of the National Research Centre

Fig. 1

From: Polymorphism of growth hormone gene in three goat breeds in Egypt

Fig. 1

Electrophoretic pattern of PCR-amplified fragment from three loci on GH gene named GH1, GH2, GH6 and their digestions. a Agarose gel electrophoresis of GH1-PCR fragment (422 bp). Lanes M (25 bp DNA ladder); 1, 2 3, 4, 5, 6 (Barki); 7, 8, 9, 11 (Damascus); and 12, 13, 14, 15, 16, 17(Zaraibi). b Agarose gel electrophoresis of GH1-HaeIII/RFLP fragments. Lane M, 25 bp DNA ladder; lanes (1, 3, 4,5, 7, 8, 9, 11, 12, 14, 15, 16, 17) genotype AB (422, 366, and 56 bp); and lanes (2, 6) genotype BB (366 and 56 bp). c Agarose gel electrophoresis of GH2-PCR fragment (116 bp). Lane M, 25 bp DNA ladder. Lanes (1, 2, 3, 4, 5) Barki; (6, 7, 8. 9, 10) Damascus; and lanes (11, 12, 13, 14, 15) Zaraibi breeds. d Agarose gel electrophoresis HaeIII/RFLP fragments. Lane M, 25  bp DNA ladder; lanes (1, 2, 3, 4, 5, 6, 7) genotype BB (88 and 28 bp); and lanes (8, 9, 10, 11) genotype AA (116 bp). e Agarose gel electrophoresis of GH6-PCR fragment (405 bp). Lane M, 25 bp DNA ladder. Lanes (1, 2, 3) Barki; (4, 5, 6) Damascus; and lanes (7, 8, 9, 10) Zaraibi breeds. f Polyacrylamide gel electrophoresis for GH6-HaeIII/RFLP. Lane M (25 bp DNA ladder) and 1 → 8 (genotype BB with 108, 78, 70, 48, 44, 40, and 17 bp)

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