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Table 1 Cultural and biochemical characteristic of the promising bacterial isolate

From: Production and optimization of α-amylase from thermo-halophilic bacteria isolated from different local marine environments

Test

Bacillus sp. NRC22017

Morphological characterization

 Gram stain

Positive

 Motility

Positive

 Spore-former

Positive

 Appearance of the colony surface

Wrinkled

 Color

Creamy

 Elevation

Umbonate

 Edge

Entire

 Whole colony

Circular

 Pigmentation

No pigment

 Opacity of the bacterial colony

Opaque

Biochemical characterization

 Starch hydrolysis

Positive

 Voges-Proskauer

Negative

 Citrate utilization

Negative

 Nitrate reduction

Negative

 Oxidase

Positive

 Catalase

Positive

Acid production from

 Glucose

Positive

 Fructose

Positive

 Galactose

Negative

 Lactose

Negative

 Maltose

Negative

 Sucrose

Positive

 Mannose

Positive

 Xylose

Negative