Strategy | Advantages | Disadvantages | References |
---|---|---|---|
Environmental stimuli | Simple, does not require molecular methods, and can be used on unexplored species | It is not specific, alters many clusters, requires genetic modifications | Reen et al. (2015) |
Global regulator | An easy method of activating several cluster activation pathways to increase the amount of metabolites | It is not specific, affects many clusters, and requires genetic modifications | |
Cluster-specific transcription factor | Targets a specific BGC, requires only one gene to be manipulated | Requires genetic modifications and requires a cluster with a transcription factor | Kjærbølling et al. (2019) |
Promoter engineering | It can activate a single BGC gene expression, can be used on full clusters, and does not require a transcription factor | Laborious, time-consuming and requires genetic modifications |