Table 2 The results of the Box–Behnken design application. The Box–Behnken design was utilized to investigate the effects of independent factors on catalase activity, which included neocuproine concentration, hydrogen peroxide concentration, and incubation time. The dependent variable, catalase activity, was assessed with the CUPRAC-CAT method. The results were obtained by averaging three replicates
From: An efficient protocol for quantifying catalase activity in biological samples
Run | Hydrogen peroxide concentration (mM) | Neocuproine concentration (mM) | Incubation time (min) | Catalase activity Katal/L |
|---|---|---|---|---|
1 | 5.5 | 10 | 3.5 | 302 |
2 | 8 | 15 | 1 | 260 |
3 | 5.5 | 18.4 | 3.5 | 301 |
4 | 8 | 15 | 6 | 281 |
5 | 3 | 15 | 1 | 189 |
6 | 5.5 | 10 | 3.5 | 300 |
7 | 5.5 | 10 | 3.5 | 305 |
8 | 8 | 5 | 6 | 270 |
9 | 3 | 5 | 6 | 238 |
10 | 1.3 | 10 | 3.5 | 292 |
11 | 3 | 5 | 1 | 240 |
12 | 5.5 | 1.6 | 3.5 | 270 |
13 | 5.5 | 10 | 7.7 | 245 |
14 | 5.5 | 10 | 3.5 | 288 |
15 | 8 | 5 | 1 | 270 |
16 | 9.7 | 10 | 3.5 | 318 |
17 | 3 | 15 | 6 | 282 |