Table 1 The procedure of the CUPRAC-CAT method
From: An efficient protocol for quantifying catalase activity in biological samples
Reagents | Test | Control | STD | Blank |
|---|---|---|---|---|
Sample | 250 μL | 250 μL | – | – |
Distilled water | – | 2000 μL | 250 μL | 2250 μL |
H2O2 | 2000 μL | – | 2000 μL | – |
The solution was vortexed and incubated at 37 °C for 2 min. Then, the reaction was terminated with 250 μl of 10% TCA. The final solution was mixed well and centrifuged for 15 min at 3000 xg. A 50 µL of supernatant was removed in a clean tube. Finally, the working solution was added | ||||
Working reagent | 3 mL | 3 mL | 3 mL | 3 mL |