Scheme 1
From: An efficient protocol for quantifying catalase activity in biological samples
The general reaction of the CUPRAC-CAT method involves using hydrogen peroxide as a substrate for CAT. The enzymatic reaction of catalase produces oxygen (O2), and water (H2O). In the presence of the Cu(II)-neocuproine complex, the reaction with the unreacted hydrogen peroxide (H2O2) results in the formation of a stable yellow-orange Cu(I)-neocuproine complex. The Cu(II)-neocuproine complex has a light blue color. The Cu(I)-neocuproine complex has a yellow color