Table 2 Sequence of primers used in real-time quantitative PCR reactions to determine the expression level of Ache gene
Gene | Primer sequence (5′ to 3′) | GenBank (accession no) |
|---|---|---|
β-actin | F: AGTCATTCAGTCATGCCCCA R: AACATACTGCCTCCGAACCA | MN044625.1 |
AChE | F:TGGCTTTGCAATGGGTGAAA R:CGGACAATGTACAGCTTCGG | KU985167.1 |