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Bulletin of the National Research Centre

Table 1 Effect of rapamycin during IVM on oocyte nuclear maturation in bovine

From: Supplementation of rapamycin during in vitro maturation promotes oocyte quality and subsequent embryo development in bovine

Rapamycin (nM)

No. of oocyte examined

Nuclear maturation stages (%)

GV

GVBD

MI

MII

0 (Control)

205

1.8 ± 0.6

4.5 ± 1.5

13.5 ± 2.2

81.2 ± 2.5a

1

205

1.1 ± 0.6

1.2 ± 0.7

7.6 ± 1.2

90.1 ± 1.5b

10

205

1.4 ± 0.5

4.8 ± 1.1

13.3 ± 1.9

82.5 ± 0.8a

100

203

1.9 ± 0.5

6.5 ± 2.2

12.5 ± 2.9

79.1 ± 3.4a

  1. Slaughter house-derived bovine oocytes were matured with different concentrations of rapamycin for 22 h, and the meiotic maturation rate was examined. IVM, in vitro maturation; GV, germinal vesicle; GVBD, germinal vesicle breakdown; MI, Metaphase I; MII, Metaphase II. Data are mean ± S.E.M. from five replicates. Means with different superscript letters (a,b) within column are significantly different (P < 0.01)
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