Table 1 Respiratory tract bacterial nested primers
S/N | Bacteria | Primer sequences (5’ → 3’) | Target |
|---|---|---|---|
1 | Staphylococcus aureus | F1-GCGATTGATGGTGATACGGTT R2-AGCCAAGCCTTGACGAACTAAAGC F2-TATGGCCCTGAAGCAAGTG R2-CGTTTACCATTTTTCCATCAGCA | Nuc |
2 | Moraxella catarrhalis | F1-CTGCCCAAGCTGCCCTAAGT R1-CAAAAGCCACAAAAACCC F2-GTGCTGGCTATCGTGTGAATCC R2-AAAAAGCAGCCAGTAAAC | uspA |
3 | Haemophilus spp. | F1-AACTTTTGGCGGTTACTCTG R1-CTAACACTGCACGACGGTTT F2-TTGGCGGATACTCTGTTGCTG R2-GTGCGCATCTAAGATTTGAACG | p6 |
4 | Chlamydophila pneumoniae | F1-TTACAAGCCTTGCCTGTAGG R1-GCGATCCCAAATGTTTAAGGC F2-TTATTAATTGATGGTACAATA R2-ATCTACGGCAGTAGTATAGTT | Momp |
5 | Mycoplasma pneumoniae | F1-ATTCTCATCCTCACCGCCACCG R1-TGGTTTGTTGACTGCCACTGCCG F2-CAATGCCATCAACCCGCGCTTAACC R2-GTTGTCGCGCACTAAGGCCCACG | p1 |