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Table 7 Phenolic compound determination in gardenia calli cultures cultured on MS media contained different concentrations of phenyl alanine or salicylic acid during the third harvested period of culturing by HPLC

From: Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid

Treatment

Phenolic compounds determined in gardenia callus cultures in μg/g dry weight in the third harvest

t. P

 

Cinnamic

Rosmarinic

P-hydroxy

Protocatechuic

Gallic

Catechin

Caffeic

Syringic

p-coumaric

Vanillic

Sinapic

Control

79.95

ND

N.D.

128.177

85.13

N.D.

N.D.

N.D.

N.D.

N.D.

N.D.

293.25

2 mg/l phenylalanine

49.87

213.16

18.76

14.2414

ND

297.23

7.14

30.71

43.47

4.86

9.0074

688.44

3 mg/l phenylalanine

92.24

ND

ND

ND

ND

ND

ND

ND

5.21

ND

ND

97.45

4 mg/l phenylalanine

49.36

40.26

19.13

ND

ND

54.72

ND

9.51

1.97

ND

ND

174.95

5 mg/l phenylalanine

68.997

15.52

10.32

ND

ND

40.28

ND

ND

5.094

ND

ND

140.21

0.25 mM salicylic acid (34.5 mg/l)

52.48

19.72

ND

ND

ND

138.59

5.35

105.9

14.87

68.23

ND

405.14

0.5 mM salicylic acid (69 mg/l)

72.37

22.48

5.21

ND

ND

180.80

ND

2.35

20.975

ND

5.4139

309.59

0.75 mM salicylic acid (103.5 mg/l)

ND

24.06

45.46

ND

ND

246.65

ND

ND

41.45

ND

8.24

365.86

1 mM salicylic acid (138 mg/l)

49.65

78.20

11.40

ND

ND

493.65

5.105

3.07

2.55

ND

6.12

649.74

  1. ND not detected