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Table 4 Flavonoids determination in gardenia calli cultures cultured on MS media contained different concentrations of phenyl alanine or salicylic acid during the three harvested periods of culturing by HPLC

From: Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid

Treatments

Flavonoids determined in gardenia callus cultures in μg/g dry weight

First harvest (after 2 weeks of culturing)

Second harvest (after 4 weeks of culturing)

Third harvest (after 5 weeks of culturing)

Rutin

App.

Kaemp.

Gent.

t. F

Ruin

App.

Kaemp.

Gent.

t.f.

Rutin

App.

Kaemp.

Gent.

t.f..

Control

22.11

ND

ND

ND.

22.11

ND

ND

ND

ND

ND

20.75

ND

ND

ND

20.75

2 mg/l phenylalanine

111.3

ND

ND

ND

111.3

77.56

ND

ND

ND

77.56

429.8

213.16

2.993

ND

645.95

3 mg/l phenylalanine

201.0

ND

ND

ND

201.0

152.0

23.22

ND

ND

175.22

8.178

8.41

1.99

ND

18.57

4 mg/l phenylalanine

20.31

ND

ND

ND

20.31

50.49

5.68

ND

ND

56.17

137.9

31.09

4.32

ND

173.31

5 mg/l phenylalanine

20.76

ND

ND

ND

20.76

119.57

ND

7.18

ND

126.75

55.91

13.73

1.66

ND

71.3

0.25 mM salicylic acid(34.5 mg/l)

96.44

ND

ND

ND

96.44

40.82

ND

4.90

ND

45.72

7.05

19.52

12.47

ND

39.04

0.5 mM salicylic acid(69mg/l)

202.2

ND

ND

ND

202.2

30.75

5.97

ND

ND

36.72

62.78

4.00

6.837

ND

73.61

0.75 mM salicylic acid(103.5mg/l)

14.06

ND

ND

ND

14.06

24.93

ND

ND

ND

24.93

75.24

N.D

3.061

3.95

82.25

1 mM salicylic acid(138mg/l)

153.4

ND

ND

ND

153.4

ND

ND

ND

ND

ND

148.2

13.47

1.88

10.36

173.91

  1. ND not detected